Coding

Part:BBa_K4825050

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


CDC9-LEU1-COX6-LEU2-COX4-LEU4^S547∆

This compostie part contains the part CDC9-LEU1 BBa_K4825033, COX6-LEU2 BBa_K4825034 and COX4-LEU4^S547∆ BBa_K4825035. In the pathway of producing isoamyl acetate, overexpression of LEU1, LEU2 and LEU4 increases the supply of precursor 2-ketoisocaproate (KIC), which can result in an overall increase in the production of isoamyl acetate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal XbaI site found at 61
    Illegal XbaI site found at 1324
    Illegal XbaI site found at 1438
    Illegal XbaI site found at 7641
    Illegal SpeI site found at 1479
    Illegal PstI site found at 4508
    Illegal PstI site found at 5003
    Illegal PstI site found at 5288
    Illegal PstI site found at 8330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal NheI site found at 1002
    Illegal SpeI site found at 1479
    Illegal PstI site found at 4508
    Illegal PstI site found at 5003
    Illegal PstI site found at 5288
    Illegal PstI site found at 8330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal BglII site found at 2209
    Illegal BamHI site found at 1155
    Illegal BamHI site found at 2121
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal XbaI site found at 61
    Illegal XbaI site found at 1324
    Illegal XbaI site found at 1438
    Illegal XbaI site found at 7641
    Illegal SpeI site found at 1479
    Illegal PstI site found at 4508
    Illegal PstI site found at 5003
    Illegal PstI site found at 5288
    Illegal PstI site found at 8330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 625
    Illegal EcoRI site found at 3427
    Illegal EcoRI site found at 4534
    Illegal EcoRI site found at 5323
    Illegal EcoRI site found at 7400
    Illegal EcoRI site found at 7864
    Illegal XbaI site found at 61
    Illegal XbaI site found at 1324
    Illegal XbaI site found at 1438
    Illegal XbaI site found at 7641
    Illegal SpeI site found at 1479
    Illegal PstI site found at 4508
    Illegal PstI site found at 5003
    Illegal PstI site found at 5288
    Illegal PstI site found at 8330
    Illegal NgoMIV site found at 4051
    Illegal AgeI site found at 3040
    Illegal AgeI site found at 6253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 911
    Illegal SapI site found at 5513
    Illegal SapI.rc site found at 895


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Categories
Parameters
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